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Journal of Chinese Society for Corrosion and protection  2019, Vol. 39 Issue (2): 176-184    DOI: 10.11902/1005.4537.2019.007
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Corrosion Mechanism of Carbon Steel Induced by Protein and Polysaccharide-the Main Components of EPS
Ping XU(),Shuo ZHANG,Shuai SI,Yajun ZHANG,Changzheng WANG
National Demonstration Center for Experimental Water Environment Education, Key Laboratory of Urban Stormwater System and Water Environment, Ministry of Education, Beijing University of Civil Engineering and Architecture, Beijing 100044, China
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Abstract  

Extracellular polymeric substance (EPS) presents certain inhibitory effect on metal corrosion, which mainly composed of protein, polysaccharide. In the study here, Bovine serum albumin (BSA) and Dextran 40 were used to represent the protein and polysaccharide respectively, with which the carbon steel was coated by dip-coating method. Thereafter, the corrosion behavior of carbon steel without and with coatings of BSA and Dextrand 40 was studied via immersion test in an artificial corrosive solution of NaCl 58.5 mg/L+Na2SO4 213 mg/L+NaHCO3 4.2 mg/L. The corrosion rate, surface morphology, corrosion products, and electrochemical behavior of the carbon steel, as well as the changes in functional groups of the BSA and Dextran 40 before and after dip-coating on carbon steel are mainly concerned in the study. Results shown that the corrosion products formed on the steel coated with BSA and Dextran 40 contained the amounts of Fe3O4 up to 113.6% and 145.5% of that on the plain steel, correspondingly, the corrosion rate decreased by 17.7%, 24.0%, respectively. The equivalent circuit of the plain steel is R(QR) and the dip-coating ones could be fitted with R(Q(R(QR))). Infrared spectroscopy revealed that, after dip-coating on the steel surface, the relevant peaks of the —C=O—, —COO— of protein, and the —C—OH, —CH3, —CH2—, —COO— of polysaccharide weakened or even disappeared, which indicated that after dip-coating a protective film may form on the surface of carbon steel. The fact implies that the functional groups of BSA and Dextran 40 may take part in the corrosion process, and play a key role on the formation of protective film.
Key words:  protein      polysaccharide      carbon steel      corrosion      protective layer      EPS     
Received:  10 January 2019     
ZTFLH:  TG172  
Fund: National Natural Science Foundation of China(51578035);Fundamental Research Funds for Beijing Universities, Research Foundation of Beijing University of Civil Engineering and Architecture(00331615008);Research Foundation of Key Laboratory of Urban Stormwater Systemand Water Environment & BUCEA(PXM2014014210000057)
Corresponding Authors:  Ping XU     E-mail:  xuping@bucea.edu.cn
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Ping XU
Shuo ZHANG
Shuai SI
Yajun ZHANG
Changzheng WANG

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Ping XU,Shuo ZHANG,Shuai SI,Yajun ZHANG,Changzheng WANG. Corrosion Mechanism of Carbon Steel Induced by Protein and Polysaccharide-the Main Components of EPS. Journal of Chinese Society for Corrosion and protection, 2019, 39(2): 176-184.

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https://www.jcscp.org/EN/10.11902/1005.4537.2019.007     OR     https://www.jcscp.org/EN/Y2019/V39/I2/176

Fig.1  Schematic diagram of experimental set-up
Fig.2  Corrosion rates of blank and dipped carbon steel in testing solution
Fig.3  SEM images (a~c) andcorresponding EDS results (d~f) of carbon steel smaples without dipping (a, d), and dipped with protein (b, e) and polysaccharide (c, f) after immersion for 16 d
Fig.4  XRD spectra of blank and dipped carbon steel samples after corrosion in testing solusion for 16 d
Fig.5  Nyquist plots of carbon steel electrode during immersion in water (a), protein (b) and polysaccharide (c) solutions
Fig.7  Equivalent circuit models for fitting EIS of carbon steel electrode during immersion in water (a) and in protein/polysaccharide solution (b)
ConditionRs / Ω·cm2Cb / μF·cm-2nbRb / Ω·cm2Cf / μF·cm-2nfRP / Ω·cm2n
Water-1 h4.67×102---------3.77×10-4---1.92×1037.15×10-1
Water-3 h4.54×102---------3.95×10-4---1.89×1037.72×10-1
Water-6 h4.24×102---------4.77×10-4---1.83×1038.09×10-1
Water-9 h4.12×102---------4.93×10-4---1.63×1038.19×10-1
Water-12 h4.39×102---------4.98×10-4---1.62×1037.56×10-1
Water-18 h5.17×102---------6.25×10-4---1.60×1037.18×10-1
Water-24 h5.27×102---------6.64×10-4---1.42×1037.90×10-1
Protein-1 h7.12×1023.24×10-88.39×10-13.08×1021.08×10-47.57×10-11.70×103---
Protein-3 h7.16×1023.78×10-88.26×10-14.17×1021.19×10-48.72×10-11.65×103---
Protein-6 h7.87×1023.81×10-89.11×10-14.29×1021.51×10-48.24×10-11.60×103---
Protein-9 h8.11×1024.06×10-89.02×10-14.72×1022.11×10-48.20×10-11.56×103---
Protein-12 h8.02×1024.30×10-89.00×10-15.35×1022.46×10-49.29×10-11.49×103---
Protein-18 h7.25×1024.46×10-89.23×10-15.35×1023.02×10-48.06×10-11.61×103---
Protein-24 h7.74×1024.96×10-89.13×10-16.23×1023.53×10-48.21×10-12.04×103---
Polysaccharide-1 h7.47×1023.36×10-89.34×10-15.25×1025.53×10-48.81×10-11.75×103---
Polysaccharide-3 h7.39×1023.45×10-88.32×10-15.31×1026.44×10-48.68×10-11.67×103---
Polysaccharide-6 h7.27×1023.53×10-87.18×10-15.50×1026.51×10-48.64×10-11.65×103---
Polysaccharide-9 h7.13×1023.66×10-88.58×10-15.63×1027.93×10-48.73×10-11.81×103---
Polysaccharide-12 h8.14×1023.69×10-88.14×10-15.87×1028.83×10-48.67×10-11.97×103---
Polysaccharide-18 h8.03×1024.10×10-89.73×10-15.97×1021.07×10-38.79×10-12.13×103---
Polysaccharide-24 h7.90×1024.70×10-89.72×10-16.08×1021.08×10-37.37×10-12.14×103---
Table 1  Fitting equivalent circuit parameters for carbon steel electrode
Fig.6  Impendance module (a, c, e) and phase angle (b, d, f) plots of carbon steel electrode during immersion in water (a, b),proteinsolution (c, d) and polysaccharide (e, f) solutions
Fig.8  FT-IR spectra of pure protein and polysaccharide powders, and corrosion products of carbon steel after immersion in protein (a) and polysaccharide (b) solutions for 24 h
Fig.9  Formation mechanism of “Chelates-corrosion products”protective layer: chelation of iron ions with protein/polysaccharide (a) and corrosion inhibition of protection layer (b)
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