海洋假交替单胞菌<i>Pseudoalteromonas lipolytica</i>分泌黑色素加速316L不锈钢腐蚀机理的研究

doi:10.11902/1005.4537.2022.131

中国腐蚀与防护学报

2022, Vol. 42

Issue (5): 743-751 DOI: 10.11902/1005.4537.2022.131

研究报告

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海洋假交替单胞菌Pseudoalteromonas lipolytica分泌黑色素加速316L不锈钢腐蚀机理的研究

郭娜, 毛晓敏, 惠芯蕊, 郭章伟, 刘涛(

)

上海海事大学海洋科学与工程学院上海 201306

Corrosion Behavior of 316L Stainless Steel in Media Containing Pyomelanin Secreted by Pseudoalteromonas lipolytica

GUO Na, MAO Xiaomin, HUI Xinrui, GUO Zhangwei, LIU Tao(

)

College of Ocean Science and Engineering, Shanghai Maritime University, Shanghai 201306, China

全文: PDF(11764 KB) HTML

摘要:

以海洋假交替单胞菌P. lipolytica的野生菌株 (WT) 与产色菌株 (∆hmgA) 为模式菌株,重点研究海洋细菌分泌黑色素加速不锈钢腐蚀的行为和机理。通过扫描电镜 (SEM)、激光共聚焦显微镜 (CLSM)、X射线衍射 (XRD) 和电化学等手段证明了WT菌株不会造成不锈钢腐蚀,不锈钢在含WT的培养液中依然会形成完整的钝化膜；而∆hmgA菌株会在分泌黑色素的同时,在不锈钢表面形成矿化位点,从而破坏了不锈钢表面的钝化膜,造成严重的点蚀。不锈钢在细菌黑色素提取液中也会形成点蚀,但由于没有细菌参与,不锈钢的钝化膜相对完整,因此点蚀程度较轻。本文研究在含有产色细菌和黑色素物质的环境条件下不锈钢的腐蚀机理以及黑色素对钝化膜形成和点蚀发展的影响机制,从而丰富和拓展产色菌和细菌色素对金属腐蚀的机理。

关键词 ：海洋假交替单胞菌, 产色菌, 细菌色素, 不锈钢, 点蚀

Abstract：

By taking marine Pseudoalteromonas lipolytica (P. lipolytica) wild type strain (WT) and the genetically engineered P. lipolytica mutant strain with hmgA gene deletion (∆hmgA) as model strains, the influence of marine bacteria secreting pyomelanin on the corrosion behavior of 316L stainless steel in marine bacteria culture solutions was studied via electrochemical measurements, scanning electron microscopy (SEM), laser confocal microscopy (CLSM) and X-ray diffraction (XRD). It was proved that WT strain did not cause corrosion of the stainless steel, correspondingly a compact passivation film may form on the steel surface in the medium containing WT. While the ∆hmgA strain will secrete pyomelanin and induce formation of mineralization sites on the stainless-steel surface, thus destroying the formed passivation film and further causing serious pitting corrosion. In bacterial pyomelanin solution, the stainless steel will also be suffered from pitting corrosion, however, a rather intact passivation film can still be formed on the steel surface due to that no bacteria may be involved to the process. In general, this study revealed that the corrosion mechanism of stainless steel in the environments containing pigmented bacteria and the influence of pyomelanin on passivation film formation and pitting development.

Key words： Pseudoalteromonas lipolytica pigmented bacteria bacterial pyomelanin stainless steel pitting

收稿日期: 2022-05-03

ZTFLH:

TG174

基金资助:国家自然科学基金(41976039);国家自然科学基金(42006039);国家自然科学基金(51901127);上海市自然科学基金(19ZR1422100)

通讯作者: 刘涛 E-mail: liutao@shmtu.edu.cn

Corresponding author: LIU Tao E-mail: liutao@shmtu.edu.cn

作者简介: 郭娜,女,1983年生,工程师

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引用本文:

郭娜, 毛晓敏, 惠芯蕊, 郭章伟, 刘涛. 海洋假交替单胞菌Pseudoalteromonas lipolytica分泌黑色素加速316L不锈钢腐蚀机理的研究[J]. 中国腐蚀与防护学报, 2022, 42(5): 743-751.
Na GUO, Xiaomin MAO, Xinrui HUI, Zhangwei GUO, Tao LIU. Corrosion Behavior of 316L Stainless Steel in Media Containing Pyomelanin Secreted by Pseudoalteromonas lipolytica. Journal of Chinese Society for Corrosion and protection, 2022, 42(5): 743-751.

链接本文:

https://www.jcscp.org/CN/10.11902/1005.4537.2022.131 或 https://www.jcscp.org/CN/Y2022/V42/I5/743

图1 P. lipolytica的野生菌 (WT)、基因突变株 (∆hmgA) 生长曲线,pH值,溶解氧在7 d内随时间变化规律

图2 不锈钢样品在不同溶液中浸泡14 d后的宏观形貌

图3 不锈钢样品分别在WT和∆hmgA培养液中浸泡不同时间并染色标记后的CLSM图像

图4 不锈钢样品分别在不同溶液浸泡14 d后的表面生物膜形貌

图5 不锈钢样品不同溶液内浸泡14 d后XRD谱

图6 不锈钢样品分别在无菌溶液、WT培养液、∆hmgA培养液和黑色素溶液浸泡14 d后白光干涉图像

图7 不锈钢样品在不同溶液浸泡14 d后的动电位极化曲线

表1 不锈钢样品在不同溶液内浸泡不同时间后极化曲线的拟合参数

图8 不锈钢样品在不同溶液内浸泡不同时间后电化学阻抗谱

表2 不锈钢样品在不同溶液内浸泡不同时间后电化学阻抗拟合参数

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