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Journal of Chinese Society for Corrosion and protection  2021, Vol. 41 Issue (6): 757-764    DOI: 10.11902/1005.4537.2020.152
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Effect of Culture Medium on Microbiologically Influenced Corrosion
LV Meiying, LI Zhenxin, DU Min(), WAN Zixuan
Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266100, China
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Abstract  

The influence of culture medium on the growth characteristics of microorganisms and their behavior in the relevant corrosion process of materials at home and abroad was introduced in detail. First, the different types of culture media were classified. The common sources and physiological functions of the main nutrient elements were summarized. The culture media for common corrosive bacteria were also briefly described. Then, the effect of different nutrient components (such as carbon source, nitrogen source and inorganic salt components, e.g., PO43-, SO42-, Cl-, Na+, K+, Ca2+ and Fe2+) on the growth characteristics and corrosion process of bacteria was emphatically discussed. It is finally prospected that the importance of compositional optimization of culture media for the clarification of the relevant mechanism of bacteria influenced corrosion.
Key words:  microbiologically influenced corrosion      culture medium      carbon source      nitrogen source      inorganic salt      growth characteristic     
Received:  18 August 2020     
ZTFLH:  Q939.98  
Fund: National Natural Science Foundation of China(51871204)
Corresponding Authors:  DU Min     E-mail:  ssdm99@ouc.edu.cn
About author:  DU Min, E-mail: ssdm99@ouc.edu.cn
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Meiying LV
Zhenxin LI
Min DU
Zixuan WAN

Cite this article: 

LV Meiying, LI Zhenxin, DU Min, WAN Zixuan. Effect of Culture Medium on Microbiologically Influenced Corrosion. Journal of Chinese Society for Corrosion and protection, 2021, 41(6): 757-764.

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https://www.jcscp.org/EN/10.11902/1005.4537.2020.152     OR     https://www.jcscp.org/EN/Y2021/V41/I6/757

Nutrient elementCommon sourcePhysiological function
CGlucose, sucrose, starch, beef extract, etc.It constitutes the cellular material of microorganisms, the skeleton of organic macromolecules, and provides energy for life activities
NYeast extract, beef extract, peptone, urea, ammonium salt, etc.It is the main element that constitutes proteins, nucleic acids, etc. In addition, ammonium and nitrate can also be used as energy sources for some bacteria
PKH2PO4, K2HPO4It is the key component of nucleic acid, nuclear protein and many coenzymes; Moreover, phosphate is an important buffer in cells
S(NH4)2SO4, MgSO4It is the component of sulfur-containing amino acids (cystine, cysteine, methionine, etc.) and sulfur-containing vitamins (biotin, thiamine, etc.); Sulfur and sulfides are also sources of energy for some autotrophic microorganisms
KKH2PO4, K2HPO4It is the cofactor of some enzymes (fructokinase, phosphopyruvate transphosphatase, etc.); It is also involved in the composition of the intracellular material transport system, and regulates membrane permeability, potential difference, and osmotic pressure
NaNaCl, NaNO3It can maintain osmotic pressure; It is essential for some bacteria and cyanobacteria
CaCa(NO3)2, CaCl2It is the stabilizer of some extracellular enzymes and the cofactor of proteases; It can also regulate the colloidal state in cells and reduce the permeability of the cell membrane
MgMgSO4It is the cofactor of nitrogenase and the component of chlorophyll; It can also stabilize ribosomes and cytoplasmic membranes
FeFeSO4It can constitute cytochrome, chlorophyll and some enzymes involved in the electron transfer process
Table 1  Source and function of main nutrients
Culture mediumKH2PO4K2HPO4NH4ClCaCl2·2H2OCaCl2·6H2OCaSO4Na2SO4FeSO4·7H2OMgSO4·7H2OSodium lactateYeast extractSodium citrate(NH4)2FeSO4·6H2O
PB0.5---1.00.06------4.50.0040.066.01.00.3---
PC---0.51.0---0.06---2.0---0.066.01.00.3---
MB---0.051.0------1.0------4.13.51.05.01.38
Table 2  Culture medium composition for SRB (g·L-1)
Culture mediumK2HPO4NaNO3CaCl2MgSO4·7H2ONH4NO3(g/L)(NH4)2SO4FeSO4·7H2OAmmonium ferric citrateKClCa(NO3)2
Winogradsky0.50.50.20.50.5------6.0------
9k0.05------0.50.15---------0.050.01
Bushnell Hass0.5------0.5---3.044.22---0.10.01
Table 3  Culture medium composition for IOB (g·L-1)
Culture mediumBeef extractYeast extractNaClPeptoneKNO3
NB3.0---5.010.0---
LB-NO3---5.05.010.010.0
Table 4  Culture medium composition for NRB (g·L-1)
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