The synergistic effect of 2,2-dibromo-3-hypoazopropionamide (DBNPA) and rhamnolipid (RL) on the corrosion behavior of X80 pipeline steel in solutions containing sulfate reducing bacteria (SRB) Desulfovibrio bizertensis SY-1 was investigated. The results showed that compared with a sterile solution, the mass loss and pitting depth of X80 pipeline steel significantly increased in the presence of Desulfovibriobizertensis SY-1, while corrosion product FeS was detected on steel surface. However, the addition of DBNPA effectively inhibited the growth of planktonic and sessile bacterial cells, thereby retarding the corrosion process on X80 pipeline steel. Notably, when 150 mg/L DBNPA and 500 mg/L RL were co-added in the solution, the corrosion rate of X80 pipeline steel decreased by 77.8% compared to that in the SRB (p = 0.009) containing solution, whilst, by 50% compared to that in the SRB containing solution with addition of 300 mg/L DBNPA alone. Furthermore, this combination also led to an approximately 84.7% reduction in corrosion current density even after 15 days' immersion compared to that in the SRB containing solution, and about 20.5% reduction compared to that in the SRB containing solution with addition of 300 mg/L DBNPA alone. Therefore, these findings found that the cooperative addition of 150 mg/L DBNPA and 500 mg/L RL can effectively inhibit the corrosion of X80 pipeline steel induced by Desulfovibrio bizertensis SY-1. The results may provide references for selecting and utilizing biocides.