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Journal of Chinese Society for Corrosion and protection  2025, Vol. 45 Issue (5): 1341-1350    DOI: 10.11902/1005.4537.2024.389
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Effect of Mo Addition on Corrosion Behavior of EH36 Steel in Seawater Included With Sulfate Reduction Bacteria
GUO Zhangwei, YE Tingyu, GUO Na, LIU Tao()
Shanghai Maritime University, College of Ocean Science and Engineering, Shanghai 201306, China
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GUO Zhangwei, YE Tingyu, GUO Na, LIU Tao. Effect of Mo Addition on Corrosion Behavior of EH36 Steel in Seawater Included With Sulfate Reduction Bacteria. Journal of Chinese Society for Corrosion and protection, 2025, 45(5): 1341-1350.

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Abstract  

In the design stage of marine engineering materials, it is easy to ignore the influence of microorganisms when the engineering facilities are in actual service. In fact, the alloying elements of the materials have a great impact on the adhesion of microorganisms and the corrosion performance of the materials. Herein, the effect of Mo addition on the corrosion behavior of EH36 marine steel in aged seawater included with sulfate reducing bacteria (SRB) is assessed via electrochemical measurement, optical microscope, scanning electron microscope and X-ray diffractometer etc. The results show that the introduction of Mo can accelerate the SRB induced corrosion of the EH36 steel, namely the thickness of the corrosion product scale of the Mo containing steel is 20 μm, while that of the steel without Mo is 13 μm, the corrosion increment of the former is as high as 40%; More pitting corrosion occurs on the surface of Mo containing steel. The relevant molecular mechanisms indicated that molybdenum could increase the expression of genes related to the adhesion and sulfate reduction processes in SRB biofilms. A denser biofilm and more hydrogen sulfide production accelerated material corrosion. Therefore, when designing materials in microbial environments, microbial factors should be fully considered.
Key words:  MIC      sulfate reducing bacteria      molybdenum      molecular mechanisms      RNA-seq     
Received:  29 November 2024      32134.14.1005.4537.2024.389
ZTFLH:  TG172  
Fund: National Natural Science Foundation of China(51901127);National Natural Science Foundation of China(41976039);National Natural Science Foundation of China(42006039)
Corresponding Authors:  LIU Tao, E-mail: liutao@shmtu.edu.cn
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GUO Zhangwei
YE Tingyu
GUO Na
LIU Tao

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https://www.jcscp.org/EN/10.11902/1005.4537.2024.389     OR     https://www.jcscp.org/EN/Y2025/V45/I5/1341

SteelCSiMnPSNbNCaMoFe
Cs0.050.21.5< 0.01< 0.0010.02< 0.0040.00250.0Bal.
Ms0.050.21.5< 0.01< 0.0010.02< 0.0040.00251.0Bal.
Table 1  Chemical compositions of the control steel (Cs) and molybdenum steel (Ms) (mass fraction / %)
Fig.1  Fluorescence microscopes of adhesions of SRB on Cs (a) and Ms (b)
Fig.2  SEM surface images of Cs (a) and Ms (b) after 14 d immersion in abiotic medium
Fig.3  SEM surface images of Cs (a) and Ms (b) after 14 d immersion in biotic medium
Fig.4  SEM cross-sectional images of Cs (a) and Ms (b) after 14 d immersion in biotic medium
Fig.5  XRD pattern of Cs (a) and Ms (b) after 14 d immersion in biotic medium containing D.vulgaris
Fig.6  EDS results of Cs (a) and Ms (b) after immersion in biotic medium for 14 d
Fig.7  2D (a, b) and 3D (c, d) images of pitting of Cs (a, c) and Ms (b, d) after immersion in abiotic medium for 14 d
Fig.8  2D (a, b) and 3D (c, d) images of pitting of Cs (a, c) and Ms (b, d) after immersion in biotic medium for 14 d
Fig.9  Size statistics of corrosion pits of Cs和Ms after 14 d immersion in abiotic medium and biotic medium
Fig.10  Corrosion rates of Ms and Cs in abiotic and biotic mediums
Fig.11  Nyquist plots of Ms (a, c) and Cs (b, d) in abiotic (a, b) and biotic (c, d) mediums, and equivalent circuit diagrams (e, f)
GroupRs / Ω·cm2Yf / S·s n ·cm-2nRfilm / Ω·cm2Ydl / S·s n ·cm-2nRct / Ω·cm2
Cs (1 d)60.796.8 × 10-50.93.6 × 1022.3 × 10-40.75.2 × 103
Cs (9 d)49.561.8 × 10-40.92.5 × 1048.4 × 10-50.92.2 × 104
Cs (14 d)56.028.0 × 10-512.4 × 1047.7 × 10-50.96.3 × 104
Ms (1 d)68.253.8 × 10-50.86.4 × 1034.5 × 10-50.92.4 × 104
Ms (9 d)69.9610.0 × 10-50.95.9 × 1031.4 × 10-30.52.9 × 102
Ms (14 d)68.141.1 × 10-40.81.7 × 1041.1 × 10-40.99.1 × 104
Table 2  Fitting parameters of EIS of Cs和Ms after immersion in the abiotic medium for different time
GroupRs / Ω·cm2Yf / S·s n ·cm-2nRfilm / Ω·cm2Ydl / S·s n ·cm-2nRct / Ω·cm2
Cs (1 d)60.203.1 × 10-50.649.23.6 × 10-50.82.9 × 104
Cs (9 d)65.701.3 × 10-30.49.7 × 1023.1× × 10-40.91.5 × 103
Cs (14 d)45.582.6 × 10-40.94.6 × 1033.4 × 10-50.593.1
Ms (1 d)64.501.7 × 10-40.923.22.9 × 10-40.98.2 × 103
Ms (9 d)59.951.1 × 10-30.617.73.4 × 10-40.91.6 × 104
Ms (14 d)61.083.1 × 10-40.722.32.0 × 10-40.96.0 × 104
Table 3  Fitting parameters of EIS of Cs和Ms after immersion in the biotic medium for different time
Fig.12  Schematic illustrations of molecular mechanism about the effect of Mo on the adhesion and corrosion of D.vulgaris on Ms: (a) gene diagram; (b) S2- synthesis related enzymes; (c) corrosion process
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