Preparation of Co3O4-Zn Composite Coating and Its Simulated Antifouling Activity of Enzymes Catalyst

doi:10.11902/1005.4537.2023.371

Journal of Chinese Society for Corrosion and protection

2024, Vol. 44

Issue (5): 1164-1176 DOI: 10.11902/1005.4537.2023.371

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Preparation of Co₃O₄-Zn Composite Coating and Its Simulated Antifouling Activity of Enzymes Catalyst

JIANG Ze¹^,²^,³, ZHAI Xiaofan²^,³(

), ZHANG Yu²^,³, SUN Jiawen²^,³, JIANG Quantong²^,³, WANG Youqiang¹(

), DUAN Jizhou²^,³(

), HOU Baorong²^,³

1 School of Mechanical and Automotive Engineering, Qingdao University of Technology, Qingdao 266520, China
2 CAS Key Laboratory of Marine Environmental Corrosion and Bio-fouling, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
3 Open Studio for Marine Corrosion and Protection, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266235, China

Cite this article:

JIANG Ze, ZHAI Xiaofan, ZHANG Yu, SUN Jiawen, JIANG Quantong, WANG Youqiang, DUAN Jizhou, HOU Baorong. Preparation of Co₃O₄-Zn Composite Coating and Its Simulated Antifouling Activity of Enzymes Catalyst. Journal of Chinese Society for Corrosion and protection, 2024, 44(5): 1164-1176.

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Abstract

Nanoparticles Co₃O₄ with peroxidase catalytic activity were co-deposited with Zn by electrodeposition to obtain a novel Co₃O₄-Zn composite coating on Q235 carbon steel. Ultrasound and sodium oleate (NaoI) were introduced during the co-deposition of Co₃O₄ and Zn, which strongly promoted dispersion and adsorption of Co₃O₄ on the co-deposited surface coating. The characterization by SEM and XRD revealed that Co₃O₄ was obviously dispersed into the Zn matrix. The addition of NaoI effectively increased the deposited amount of Co₃O₄ in the Co₃O₄-Zn coatings. Besides, the antimicrobial performance of the Co₃O₄-Zn coatings was evaluated with three typical fouling bacteria, namely, Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), and Pseudomonas aeruginosa (P. aeruginosa). Results showed that the coverage of these three bacteria on Co₃O₄-Zn coatings decreased over 98%, illustrating that the Co₃O₄-Zn coatings showed high and broad-spectrum antimicrobial performance. It was also found that the Co₃O₄-Zn coating in the presence of hydrogen peroxide (H₂O₂) produced superoxide radicals (·O $2 -$ ) and hydroxyl radicals (·OH), which played dominant roles in the antimicrobial process. Finally, the antimicrobial stability and corrosion resistance of the composite coatings were also verified, and it was found that the composite coatings exhibited good antimicrobial stability and corrosion resistance characteristics. The results of this study provide a new possibility for the development of bactericidal coatings of simulated enzyme catalyst and a new solution for green antifouling.

Key words: Co₃O₄-Zn coating ultrasound assistance simulated enzyme-catalyzed antimicrobial electrodeposition sodium oleate

Received: 21 November 2023 32134.14.1005.4537.2023.371

ZTFLH:

TG174

Fund: National Natural Science Foundation of China(42376204);Shandong Provincial Natural Science Foundation(ZR2022MD023);Wenhai Program of the S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology(2021WHZZB2303);the Young Elite Scientists Sponsorship Program by CAST(YESS20210201)

Corresponding Authors: ZHAI Xiaofan, E-mail: zhaixf@qdio.ac.cnDUAN Jizhou, E-mail: duanjz@qdio.ac.cn

About author: First author contact:WANG Youqiang, E-mail: wyq1970301@126.com

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	JIANG Ze
	ZHAI Xiaofan
	ZHANG Yu
	SUN Jiawen
	JIANG Quantong
	WANG Youqiang
	DUAN Jizhou
	HOU Baorong

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https://www.jcscp.org/EN/10.11902/1005.4537.2023.371 OR https://www.jcscp.org/EN/Y2024/V44/I5/1164

Table 1 Electrodeposition parameters of Co₃O₄-Zn composite coatings

Fig.1 SEM images of ZB (a), ZY (b), ZC (c) and ZCY (d) composite coatings

Fig.2 SEM images of Co₃O₄ powders (a) and ZCY coating (b), and corresponding EDS element mappings of ZCY coating (c-f)

Fig.3 Cross-sectional morphology of ZCY coating (a) and elemental mappings in the region 1 (b), and SEM image of the region 2 in Fig.3b (c) and EDS line scannings along the line in Fig.3c (d)

Table 2 EDS determined contents of Co and Zn in the composite coatings

Fig.4 XRD patterns of the coatings, showing the exitance of Co₃O₄ (a) and Zn (b)

Fig.5 Electrochemical analysis results of ZB, ZY, ZC and ZCY coatings during electrodeposition (a-e), and equivalent circuit for fitting EIS data (f)

Fig.6 Fluorescence microscope images of ZB, ZY, ZC and ZCY coatings after immersion for 2 h in Escherichia coli solutions with and without the addition of H₂O₂ (a), and the corresponding histograms of bacterial coverage on the coatings (b)

Fig.7 Fluorescence microscopy images of ZB and ZCY coatings after immersion for 2 h in Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa solutions with the addition of hydrogen peroxide (a), and corresponding histograms of bacterial coverage (b)

Fig.8 Fluorescence microscopy images (a) and bacterial coverages (b) of ZB and ZCY composite coatings in Escherichia coli solution during cyclic immersion for 8 cycles

Fig.9 Nyqusit plots (a), R_ct values (b), Tafel curves (c) of ZB and ZCY coatings in seawater, and corresponding equivalent circuit diagram (d)

Table 3 Calculated self-etching potentials and self-etching current densities of ZB and ZCY coatings in seawater based on Tafel curves in Fig.9c

Fig.10 Fluorescence microscopy images of ZB and ZCY coatings after adding scavenger in the absence and addition of H₂O₂ (a), and corresponding histograms of bacterial coverage (b), and EPR spectra of $⋅ O 2 -$ , h⁺, and $⋅ O H$ for ZCY coating (c-e)

Fig.11 Schematic diagram of bactericidal mechanism of Co₃O₄-Zn composite coatings

Fig.12 Bactericidal conditions of cobalt tetroxide powders in various bacteria solutions without and with H₂O₂ after the addition of scavenger

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