嗜硫小红卵菌对寡营养环境中硫酸盐还原菌腐蚀的抑制作用研究

doi:10.11902/1005.4537.2025.109

中国腐蚀与防护学报

2026, Vol. 46

Issue (2): 430-440 CSTR: 32134.14.1005.4537.2025.109 DOI: 10.11902/1005.4537.2025.109

研究报告

本期目录 | 过刊浏览

嗜硫小红卵菌对寡营养环境中硫酸盐还原菌腐蚀的抑制作用研究

张进凯¹^,², 郭定¹^,², 杨金峰¹^,², 王亚楠¹(

), 段继周¹(

)

^1.中国科学院海洋研究所海洋关键材料全国重点实验室青岛 266071
^2.中国科学院大学北京 100049

Corrosion Inhibition of Rhodovulum Sulfidophilum Against Sulfate-reducing Bacteria in Oligotrophic Seawater Environment

ZHANG Jinkai¹^,², GUO Ding¹^,², YANG Jinfeng¹^,², WANG Yanan¹(

), DUAN Jizhou¹(

)

^1.Key Laboratory of Advanced Marine Materials, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
^2.University of Chinese Academy of Science, Beijing 100049, China

引用本文:

张进凯, 郭定, 杨金峰, 王亚楠, 段继周. 嗜硫小红卵菌对寡营养环境中硫酸盐还原菌腐蚀的抑制作用研究[J]. 中国腐蚀与防护学报, 2026, 46(2): 430-440.
Jinkai ZHANG, Ding GUO, Jinfeng YANG, Yanan WANG, Jizhou DUAN. Corrosion Inhibition of Rhodovulum Sulfidophilum Against Sulfate-reducing Bacteria in Oligotrophic Seawater Environment[J]. Journal of Chinese Society for Corrosion and protection, 2026, 46(2): 430-440.

全文: PDF(11542 KB) HTML

摘要:

通过电化学分析和表面形貌观察，系统研究嗜硫小红卵菌、硫酸盐还原菌(SRB)及其混合菌群在寡营养海水环境中对EH40钢腐蚀行为的影响，并揭示了种间电子传递现象在腐蚀过程中的作用机制。结果表明，嗜硫小红卵菌能够以硫代硫酸钠为电子供体，在厌氧海水中进行光合自养生长，从而减缓EH40钢的腐蚀。相比之下，单独存在的SRB则会引起EH40钢显著的腐蚀失重和点蚀。然而，当两者共培养时，混合菌群明显抑制了EH40钢的电化学反应速率，降低了SRB引起的腐蚀失重和局部点蚀。线性扫描伏安(LSV)曲线和电流-时间(I-t)曲线测试进一步证实，嗜硫小红卵菌和SRB之间存在种间电子传递过程。SRB能够利用嗜硫小红卵菌产生的光电子，而不再直接从EH40钢表面夺取电子，从而减轻了SRB引起的电化学腐蚀。种间电子转移在微生物协同作用下对钢材腐蚀具有重要调控作用。

关键词 ：微生物腐蚀, 嗜硫小红卵菌, 硫酸盐还原菌, 种间电子传递, 寡营养环境

Abstract：

The corrosion behavior of EH40 steel in oligotrophic seawater environments inoculated with R. sulfidophilum, sulfate-reducing bacteria (SRB), and their mixed consortia was assessed by means of electrochemical tests and surface morphology analyses. Particular attention was given to the role of interspecies electron transfer in modulating corrosion. Results show that R. sulfidophilum can utilize sodium thiosulfate as an electron donor to enable photoautotrophic growth in anaerobic conditions, thereby mitigating the corrosion of EH40 steel. In contrast, SRB alone induced severe mass loss and localized pitting. However, when co-cultured, the mixed community significantly suppressed the electrochemical reaction rate of EH40 steel, reducing SRB-induced corrosion damage. Linear sweep voltammetry (LSV) and current-time (I-t) measurements confirmed the occurrence of extracellular electron transfer between R. sulfidophilum and SRB. By harvesting photogenerated electrons released from R. sulfidophilum, SRB were less dependent on directly extracting electrons from the steel surface, thereby, alleviated the electrochemical corrosion. This study highlights the regulatory role of interspecies electron transfer in microbial interactions governing the steel corrosion.

Key words： microbiologically influenced corrosion R. sulfidophilum sulfate reducing bacteria interspecies electron transfer oligotrophic environments

收稿日期: 2025-04-03 32134.14.1005.4537.2025.109

ZTFLH:

TG174

基金资助:国家自然科学基金(42076044);国家自然科学基金(42206126);山东省自然科学基金(ZR2021QD099)

通讯作者: 王亚楠，E-mail：wangyanan@qdio.ac.cn，研究方向为海洋微生物腐蚀;
段继周，E-mail：duanjz@qdio.ac.cn，研究方向为海洋微生物腐蚀机制与防护技术

作者简介: 张进凯，男，1999年生，硕士生

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https://www.jcscp.org/CN/10.11902/1005.4537.2025.109 或 https://www.jcscp.org/CN/Y2026/V46/I2/430

图1 接种SOB、SRB和混合菌群的培养基中pH值变化

图2 EH40钢在SOB、SRB和混合菌群培养基中浸泡14 d后的试片表面SEM形貌

图3 EH40钢分别在SOB、SRB和混合菌群培养基中浸泡14 d后的荧光染色显微镜图

图4 EH40钢在含有SOB、SRB和混合菌群培养基中浸泡14 d后的失重速率

图5 EH40钢在SOB、SRB和混合菌群培养基中浸泡14 d后的最大点蚀坑深度

图6 在接种SOB、SRB和混合菌群的培养基中浸泡14 d后EH40钢表面腐蚀产物的Fe 2p和O 1s的XPS窄扫描光谱、宽扫描光谱以及Raman光谱

图7 EH40钢在不同接种物培养基中浸泡14 d的电化学测试结果

图8 EH40钢在接种SOB、SRB和混合接种的培养基中浸泡14 d的EIS谱

表1 EH40钢在接种SOB、SRB和混合接种的培养基中浸泡14 d后的动电位极化曲线的拟合参数

图9 SRB和SOB的LSV曲线和混合细菌体系的I-t曲线

图10 嗜硫小红卵菌对SRB的缓蚀机理示意图

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