海洋环境中硫酸盐还原菌的快速测定方法研究

doi:10.11902/1005.4537.2019.161

中国腐蚀与防护学报

2019, Vol. 39

Issue (5): 387-394 DOI: 10.11902/1005.4537.2019.161

本期目录 | 过刊浏览

海洋环境中硫酸盐还原菌的快速测定方法研究

戚鹏^1,^2,³,万逸^1,^2,³,曾艳^1,^2,³,郑来宝^1,^2,³,张盾^1,^2,³(

)

1. 中国科学院海洋研究所中国科学院海洋环境腐蚀与生物污损重点实验室青岛 266071
2. 中国科学院海洋大科学研究中心青岛 266071
3. 青岛海洋科学与技术国家实验室海洋腐蚀与防护开放工作室青岛 266237

Rapid Detection Methods for Sulfate-reducing Bacteria in Marine Environments

QI Peng^1,^2,³,WAN Yi^1,^2,³,ZENG Yan^1,^2,³,ZHENG Laibao^1,^2,³,ZHANG Dun^1,^2,³(

)

1. Key Laboratory of Marine Environmental Corrosion and Bio-fouling, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
2. Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China
3. Open Studio for Marine Corrosion and Protection, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China

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摘要:

硫酸盐还原菌 (SRB) 是腐蚀性最强，也是研究最广泛的腐蚀微生物，广泛存在于海洋环境中。SRB的状态与其参与的腐蚀过程密切相关，而SRB状态的快速测定仍是一大难题。SRB种群浓度和代谢活性的快速、准确测定，能够为揭示SRB在腐蚀过程中的作用机理，实现微生物腐蚀状态监测提供理论依据和指导。本文将简要介绍本课题组在SRB种群浓度和代谢活性检测的研究进展，阐述各类方法的优缺点、适用条件及检测性能，为后续SRB状态检测方法的发展及实用化提供参考。

关键词 ：硫酸盐还原菌, 活性, 种群浓度, 测定

Abstract：

Sulfate-reducing bacteria (SRB) are the most corrosive and the most extensively studied corrosion related microorganisms, they are widely distributed in various marine environments. Although it has been clearly known that the status of SRB is closely related with their roles in relevant corrosion processes, however the determination of the population concentration and metabolic activity of SRB is still a big problem in the present. The development of quick and accurate detection approach could provide theoretical basis and guidance for revealing the mechanism of SRB action in the corrosion process and monitoring of microbial influenced corrosion status. This paper briefly introduces the research progress of our research group in detection of the population concentration and metabolic activity of SRB, and elaborates their advantages and disadvantages, applicable conditions and detection performance, which may provide reference for the development and practical application of detection methods for the actual situation of SRB in marine environments in the future.

Key words： sulfate-reducing bacteria metabolic activity bacterial population detection

收稿日期: 2019-09-18

ZTFLH:

TM207

基金资助:国家自然科学基金(41876101);中国科协青年人才托举工程(2018QNRC001)

通讯作者: 张盾 E-mail: zhangdun@qdio.ac.cn

Corresponding author: Dun ZHANG E-mail: zhangdun@qdio.ac.cn

作者简介: 戚鹏，男，1986年生，博士，副研究员

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引用本文:

戚鹏, 万逸, 曾艳, 郑来宝, 张盾. 海洋环境中硫酸盐还原菌的快速测定方法研究[J]. 中国腐蚀与防护学报, 2019, 39(5): 387-394.
QI Peng, WAN Yi, ZENG Yan, ZHENG Laibao, ZHANG Dun. Rapid Detection Methods for Sulfate-reducing Bacteria in Marine Environments. Journal of Chinese Society for Corrosion and protection, 2019, 39(5): 387-394.

链接本文:

https://www.jcscp.org/CN/10.11902/1005.4537.2019.161 或 https://www.jcscp.org/CN/Y2019/V39/I5/387

图1 多巴胺自激发检测平台的构建流程及检测性能评价[18]

图2 氧化石墨烯标记检测平台的构建流程及检测性能评价[21]

图3 基于生物印迹薄膜检测平台的构建示意图及检测性能图[24]

图4 基于巯基蛋白酶抑制作用检测SRB的原理及性能图[27]

图5 基于ZnO/ZnS阵列转化检测平台检测SRB的原理图及检测性能[32]

图6 基于利用DNA纳米生物条码-荧光系统的检测微生物原理图及检测性能图[35]

图7 基于新型酶标体系信号放大检测平台检测微生物原理图[36]

图8 全固态硫离子选择性电极的离子传导示意图及SRB生物膜代谢活性监测性能

图9 荧光探针测定SRB生物膜表面种群浓度和生物膜代谢活性

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