Please wait a minute...
Journal of Chinese Society for Corrosion and protection  2019, Vol. 39 Issue (6): 484-494    DOI: 10.11902/1005.4537.2018.164
Current Issue | Archive | Adv Search |
Influence of Magnetic Field on Corrosion of Pure Cu in Artificial Seawater with Multispecies Aerobic Bacteria
WEI Xiaoyang1,2,MORADI Masoumeh2,YANG Lijing2,LV Zhanpeng1,ZHENG Bizhang2,SONG Zhenlun2()
1. School of Materials Science and Engineering, Shanghai University, Shanghai 200072, China
2. Key Laboratory of Marine Materials and Related Technologies, Zhejiang Key Laboratory of Marine Materials and Protective Technologies, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo 315201, China
Download:  HTML  PDF(9719KB) 
Export:  BibTeX | EndNote (RIS)      
Abstract  

The effect of static permanent magnetic fields of 28 and 60 mT on the growth rate of marine aerobic bacteria isolated from the East China Sea is studied. The corrosion behavior of pure Cu was also investigated in the artificial seawater with marine aerobic isolated bacteria in the presence of magnetic field by means of electrochemical measurement techniques and surface analysis methods. The formation of biofilm on Cu surface was observed by confocal laser scanning microscopy (CLSM). CLSM images showed that the formation and falling off of the biofilm were accelerated in the presence of magnetic field,while the effect of 60 mT magnetic field was stronger than that of 28 mT. FTIR analysis confirmed that the biofilm structures were changed when magnetic field was introduced to the system, the composition of biofilm changed from lipids, proteins, and carbohydrates to proteins and carbohydrates, while, the amount of protein decreased, but that of the carbohydrate increased. Besides, with the increase of magnetic field intensity, the lipid content decreased. The results of XPS analysis further confirmed that magnetic field affected the nature of corrosion products. The pitting was observed on the Cu surface after removing the formed biofilm using FE-SEM, nevertheless the number and size of pits on the Cu were markedly decreased in the presence of magnetic field. EIS results showed the impedance of pure Cu was significantly increased in the presence of magnetic field. It is concluded that magnetic field could accelerated the formation and falling off of the biofilm by affecting its composition and structure, therewith, inhibit the microbial corrosion process of pure Cu.

Key words:  multispecies marine bacteria      magnetic field      microbiologically-influenced corrosion     
Received:  07 November 2018     
ZTFLH:  TG172.9  
Fund: Supported by Natural Science Foundation of Ningbo(2018A610211);Ningbo 135 Marine Economic Innovation and Development Demonstration Project(NBHY-2017-Z2)
Corresponding Authors:  Zhenlun SONG     E-mail:  songzhenlun@nimte.ac.cn

Cite this article: 

WEI Xiaoyang,MORADI Masoumeh,YANG Lijing,LV Zhanpeng,ZHENG Bizhang,SONG Zhenlun. Influence of Magnetic Field on Corrosion of Pure Cu in Artificial Seawater with Multispecies Aerobic Bacteria. Journal of Chinese Society for Corrosion and protection, 2019, 39(6): 484-494.

URL: 

https://www.jcscp.org/EN/10.11902/1005.4537.2018.164     OR     https://www.jcscp.org/EN/Y2019/V39/I6/484

Fig.1  Growth curves of bacterias in the presence and absence of magnetic fields
Fig.2  SEM images of pure copper immersed in bacteria solution under 0 (a1~d1), 28 mT (a2~d2) and 60 mT (a3~d3) magnetic fields for 1 d (a1~a3), 3 d (b1~b3), 7 d (c1~c3) and 10 d (d1~d3)
Fig.3  SEM images of pure copper after removing corrosion products formed during immersion for 10 d in bacteria solution under the magnetic fields of 0 (a), 28 (b) and 60 (c) mT
Fig.4  CLSM images of pure copper immersed in bacteria solution under 0 (a, d, g), 28 mT (b, e, h) and 60 mT (c, f, i) magnetic fields for 1 d (a~c), 7 d (d~f) and 10 d (g~i)
Fig.5  FTIR spectra of the biofilms formed on pure copper immersed in bacteria solution for 10 d under the magnetic fields of 0, 28 and 60 mT
Fig.6  High resolution XPS spectra of O 1s (a), C 1s (b), N 1s (c) and Cu 2p (d) for pure copper after immersion in bacteria solution for 10 d under the magnetic fields of 0, 28 and 60 mT
Valence stateSample surfaceBinding energy / eVProposed componentsAtomicfraction / %
O 1sBacteria531.3Cu2O17.86
530.2Cu2O
Bacteria+28 mT529.1CuO19.73
530.5Cu2O
Bacteria+60 mT532.0C=O25.79
533.2C-O
C 1sBacteria283.9C=C73.88
285.4C=N
287.7C=O/CO2
Bacteria+28 mT282.2C-O66.05
283.7C=C
285.6C-N
Bacteria+60 mT285.0C-C60.04
285.8C=N
N 1sBacteria399.1=N-3.11
400.3C-NH2
Bacteria+28 mT398.0=N-4.95
399.2C-N-C
Bacteria+60 mT400.1C-NH25.18
401.3N=Cu
Cu 2pBacteria933.1Cu2O3.01
934.9CuO
942.4CuO
953.7Cu2O
962.1Cu2+
Bacteria+28 mT931.9Cu/Cu2O5.02
933.8CuO
941.8CuO
953.2Cu2O
961.6Cu2O
Bacteria+60 mT935.1CuO5.47
942.2CuO
944.3CuO
955.5Cu2+
963.2Cu2O
Table 1  Fitting parameters of C1s, O1s, N1s and Cu2p XPS spectra and the relative contents of compounds in the outermost corrosion product films on pure copper after immersion for 10 d under the magnetic fields of 0, 28 and 60 mT
Fig.7  Polarization curves of pure copper after immersion in bacteria-bearing seawater for 10 d under the magnetic fields of 0, 28 and 60 mT
SampleEcorr / V (vs SCE)Icorr / μA·cm-2βc / mV·dec-1βa / mV·dec-1Corrosion rate / 10-3 mm·a-1
Without MF-0.2691.740-758525.50
With 28 mT MF-0.3160.637-53589.34
With 60 mT MF-0.3080.559-67538.21
Table 2  Polarization parameters for pure copper immersed in bacteria-bearing seawater for 10 d under the magnetic fields of 0, 28 and 60 mT
Fig.8  Nyquist (a~c) and Bode (e~f) plots of pure copper immersed in bacteria solution under the magnetic fields of 0 (a, d), 28 mT (b, e) and 60 mT (c, f)
Fig.9  Equivalent circuits of EIS for pure copper after immersion in seawater with (a) and without (b) magnetic fields for 10 d
Magnitic field / mTTimedRsΩ·cm2Rct±SDΩ·cm2CPEdlμF·cm-2Rp±SDΩ·cm2CPEpμF.cm-2Rf ±SDΩ·cm2CPEfμF·cm-2Rb±SDΩ·cm2CPEbμF·cm-2
004.619819±1.3627.5365.9±0.7237.1------------
15.6219650±5.7054.4501.1±0.4578------------
32.6119720±4.2112.6389.0±0.9154.3------------
53.5617430±4.1863.3462.5±0.958.8------------
73.8511610±2.4520.1206.2±0.8384.4------------
103.257259±1.1122.5216.7±0.7898.6------------
2804.469294±1.3172.5------233.9±1.2737.175.28±0.186.8
16.9434210±1.7865.5------312.5±1.12153.0292.20±0.2129.4
33.1042200±3.5612.0------4314.0±1.87251.0379.30±0.094.7
52.9741250±6.92112.0------2108.0±0.981170.0584.60±0.87210.0
72.6921580±4.0285.7------8864.0±2.35310.08.51±0.0385.7
102.9314170±1.64106.0------7301.0±1.332800.046.30±0.05106.0
6004.459248±1.2775.6------232.8±1.3217.275.28±0.156.8
16.2435240±1.3071.9------1740.0±1.4525.2281.60±0.2143.3
34.7441020±3.8926.1------902.9±1.6626.956.78±0.6522.9
53.2523020±4.89105.0------6972.0±1.02134.048.07±0.0939.9
74.7719220±1.93121.0------5467.0±2.30162.016.00±0.0441.6
101.8716570±1.67136.0------4337.0±1.41332.03.42±0.0152.2
Table 3  Impedance parameters of pure copper after immersion in seawater for different time in the absence and presence of magnitic fields
[1] Drach A, Tsukrov I, DeCew J, et al. Field studies of corrosion behaviour of copper alloys in natural seawater [J]. Corros. Sci., 2013, 76: 453
[2] Chen S Q, Wang P, Zhang D. Corrosion behavior of copper under biofilm of sulfate-reducing bacteria [J]. Corros. Sci., 2014, 87: 407
[3] Chen S Q, Zhang D. Study of corrosion behavior of copper in 3.5wt.%NaCl solution containing extracellular polymeric substances of an aerotolerant sulphate-reducing bacteria [J]. Corros. Sci., 2018, 136: 275
[4] Huttunen-Saarivirta E, Rajala P, Bomberg M, et al. EIS study on aerobic corrosion of copper in ground water: influence of micro-organisms [J]. Electrochim. Acta, 2017, 240: 163
[5] San N O, Naz?r H, D?nmez G. Microbially influenced corrosion and inhibition of nickel-zinc and nickel-copper coatings by Pseudomonas aeruginosa [J]. Corros. Sci., 2014, 79: 177
[6] San N O, Naz?r H, D?nmez G. Microbial corrosion of Ni-Cu alloys by Aeromonas eucrenophila bacterium [J]. Corros. Sci., 2011, 53: 2216
[7] Fijalkowski K, Nawrotek P, Struk M, et al. The effects of rotating magnetic field on growth rate, cell metabolic activity and biofilm formation by staphylococcus aureus and escherichia coli [J]. J. Magn., 2013, 18: 289
[8] Stra?ák L, Vetterl V, ?marda J. Effects of low-frequency magnetic fields on bacteria Escherichia coli [J]. Bioelectrochemistry, 2002, 55: 161
[9] Zhang P, Zhu Q, Su Q, et al. Corrosion behavior of T2 copper in 3.5% sodium chloride solution treated by rotating electromagnetic field [J]. Trans. Nonferrous Met. Soc. China, 2016, 26: 1439
[10] Lu Z P, Yang W. In situ monitoring the effects of a magnetic field on the open-circuit corrosion states of iron in acidic and neutral solutions [J]. Corros. Sci., 2008, 50: 510
[11] Hinds G, Coey J M D, Lyons M E G. Influence of magnetic forces on electrochemical mass transport [J]. Electrochem. Commun., 2001, 3: 215
[12] Sueptitz R, Tschulik K, Uhlemann M, et al. Effect of high gradient magnetic fields on the anodic behaviour and localized corrosion of iron in sulphuric acid solutions [J]. Corros. Sci., 2011, 53: 3222
[13] Legeai S, Chatelut M, Vittori O, et al. Magnetic field influence on mass transport phenomena [J]. Electrochim. Acta, 2004, 50: 51
[14] Koza J A, Mühlenhoff S, ?abiński P, et al. Hydrogen evolution under the influence of a magnetic field [J]. Electrochim. Acta, 2011, 56: 2665
[15] Li J N, Zhang P, Guo B. Effects of rotating electromagnetic on flow corrosion of copper in seawater [J]. Trans. Nonferrous Met. Soc., 2011, 21(Suppl.2): S489
[16] Xu Y B, Hou M Y, Ruan J J, et al. Effect of magnetic field on surface properties of Bacillus cereus CrA and its Extracellular Polymeric Substances (EPS) [J]. J. Adhes. Sci. Technol., 2014, 28: 2196
[17] Zheng B J, Li K J, Liu H F, et al. Effects of magnetic fields on microbiologically influenced corrosion of 304 stainless steel [J]. Ind. Eng. Chem. Res., 2013, 53: 48
[18] Liu H W, Gu T Y, Zhang G A, et al. The effect of magneticfield on biomineralization and corrosion behavior of carbon steel induced by iron-oxidizing bacteria [J]. Corros. Sci., 2016, 102: 93
[19] Burmolle M, Ren D W, Bjarnsholt T, et al. Interactions in multispecies biofilms:do they actually matter? [J]. Trends Microbiol., 2014, 22: 84
[20] Zengler K, Palsson B O. A road map for the development of community systems (CoSy) biology [J]. Nat. Rev. Microbiol., 2012, 10: 366
[21] Batmanghelich F, Li L, Seo Y. Influence of multispecies biofilms of Pseudomonas aeruginosa and Desulfovibrio vulgaris on the corrosion of cast iron [J]. Corros. Sci., 2017, 121: 94
[22] Kester D R, Duedall I W, Connors D N, et al. Preparation of artificial seawater [J]. Limnol. Oceanogr., 1967, 12: 176
[23] Jackson M, Mantsch H H. The use and misuse of FTIR spectroscopy in the determination of protein structure [J]. Crit. Rev. Biochem. Mol. Boil, 1995, 30: 95
[24] Morikawa M. Beneficial biofilm formation by industrial bacteria Bacillus subtilis and related species [J]. J. Biosci. Bioeng., 2006, 101: 1
[25] Peng X B, Li Q, Ou L N, et al. GC-MS, FT-IR analysis of black fungus polysaccharides and its inhibition against skin aging in mice [J]. Int. J. Biol. Macromol., 2010, 47: 304
[26] Zhao S S, Cao F S, Zhang H, et al. Structural characterization and biosorption of exopolysaccharides from Anoxybacillus sp. R4-33 isolated from radioactive radon hot spring [J]. Appl. Biochem. Biotechnol., 2014, 172: 2732
[27] Schmitt J, Flemming H C. FTIR-spectroscopy in microbial and material analysis [J]. Int. Biodeterior. Biodegrad., 1998, 41: 1
[28] Zhang D Q, Goun Joo H, Lee Y K. Investigation of molybdate-benzotriazole surface treatment against copper tarnishing [J]. Surf. Interface Anal., 2009, 41: 164
[29] Xu Y J, Weinberg G, Liu X, et al. Nanoarchitecturing of activated carbon: Facile strategy for chemical functionalization of the surface of activated carbon [J]. Adv. Funct. Mater., 2008, 18: 3613
[30] Landoulsi J, Genet M J, Fleith S, et al. Organic adlayer on inorganic materials: XPS analysis selectivity to cope with adventitious contamination [J]. Appl. Surf. Sci., 2016, 383: 71
[31] Vassallo E, Cremona A, Ghezzi F, et al. Structural and optical properties of amorphous hydrogenated silicon carbonitride films produced by PECVD [J]. Appl. Surf. Sci., 2006, 252: 7993
[32] Chang F H, Chen T Y, Lee S H, et al. Corrosion inhibition of copper particles on ITO with 1,2,4-triazole-3-carboxylic acid [J]. Surf. Interface Anal., 2018, 10: 162
[33] Akhavan O, Azimirad R, Safa S, et al. CuO/Cu(OH)2 hierarchical nanostructures as bactericidal photocatalysts [J]. J. Mater. Chem., 2011, 21: 9634
[34] Haverkamp R G, Siew D C W, Barton T F. XPS study of the changes during the service life of polyester powder coatings [J]. Surf. Interface Anal., 2002, 33: 330
[35] Chen Z G, Zou J, Liu Q F, et al. Self-assembly and cathodoluminescence of microbelts from Cu-doped boron nitride nanotubes [J]. ACS Nano, 2008, 2: 1523
[36] Zhang P, Guo B, Jin Y P, et al. Corrosion characteristics of copper in magnetized sea water [J]. Trans. Nonferrous Met. Soc. China, 2007, 17: S189
[37] Javed M A, Stoddart P R, Palombo E A, et al. Inhibition or acceleration: Bacterial test media can determine the course of microbiologically influenced corrosion [J]. Corros. Sci., 2014, 86: 149
[38] Chongdar S, Gunasekaran G, Kumar P. Corrosion inhibition of mild steel by aerobic biofilm [J]. Electrochim. Acta, 2005, 50: 4655
[39] Flis J, Zakroczymski T. Impedance study of reinforcing steel in simulated pore solution with tannin [J]. J. Electrochem. Soc., 1996, 143: 2458
[40] Juzeliūnas E, Ramanauskas R, Lugauskas A, et al. Microbially influenced corrosion acceleration and inhibition. EIS study of Zn and Al subjected for two years to influence of Penicillium frequentans, Aspergillus niger and Bacillus mycoides [J]. Electrochem. Commun., 2005, 7: 305
[41] Guo B, Zhang P, Jin Y P, et al. Effects of alternating magnetic field on the corrosion rate and corrosion products of copper [J]. Rare Met., 2008, 27: 324
[1] Kangnan ZHANG,Ming WU,Fei XIE,Dan WANG,Yuxi SAN,Feng JIANG. Effect of Magnetic Field on Corrosion of X80 Pipeline Steel in Meadow Soil at Shenyang Area[J]. 中国腐蚀与防护学报, 2017, 37(2): 148-154.
[2] Yalin LV,Bijuan ZHENG,Hongwei LIU,Fuping XIONG,Hongfang LIU,Yulong HU. Effect of Static Magnetic Field on Adhesion of Sulfate Reducing Bacteria Biofilms on 304 Stainless Steel[J]. 中国腐蚀与防护学报, 2016, 36(6): 652-658.
[3] CHEN Bi, ZHENG Bijuan, ZHANG Fan, LIU Hongfang. Corrosion Behavior of HSn70-1 Copper Alloy in SRB Containing Medium in Atatic Magnetic Field[J]. 中国腐蚀与防护学报, 2014, 34(4): 339-345.
[4] LI Kejuan,ZHENG Bijuan,CHEN Bi,LIU Hongfang. Effect of Magnetic Field on Microbiologically-influenced Corrosion Behavior of Q235 Steel[J]. 中国腐蚀与防护学报, 2013, 33(6): 463-469.
[5] LI Jian ZHANG Tao MENG Guozhe SHAO Yawei WANG Fuhui. STOCHASTIC ANALYSIS OF THE MAGNETIC FIELD INFLUENCE ON THE PITTING MECHANISM OF PURE MAGNESIUM[J]. 中国腐蚀与防护学报, 2009, 29(6): 405-410.
[6] Zhanpeng Lv; Wu Yang; Delun Huang. OPEN CIRCUIT STATE AND CATHODIC DIFFUSION PROCESS OF COPPER IN SULFURIC ACID SOLUTION CONTAINING FERRIC ION INTHE PRESENCE OF MAGNETIC FIELD WITH DIFFERENT INTENSITY[J]. 中国腐蚀与防护学报, 2001, 21(3): 129-136 .
[7] Zhanpeng Lv; Delun Huang; Wu Yang. EFFECT OF MAGNETIC FIELD AND DICHROMATE ONANODIC POLARIZATION BEHAVIOR OF IRON IN SULFURIC ACID[J]. 中国腐蚀与防护学报, 2001, 21(1): 1-9 .
[8] Zhanpeng Lv. EFFECT OF MAGNETIC FIELD ON OPEN CIRCUIT CORROSION AND POLARIZATION RESISTANCE FOR IRON IN SULFURIC ACID CONTAINING DICHROMATE[J]. 中国腐蚀与防护学报, 2000, 20(4): 230-236 .
[9] CHEN Jun-ming LU Zhan-peng (Shanghai Institute of Metallurgy; Chinese Academy of Science; Shanghai 200050). ANODIC POLARIZATION BEHAVIOR OF Fe/Na_2SO_4 SYSTEM IN THE PRESENCE OF MAGNETIC FIELD AND ADDITIONAL IONS[J]. 中国腐蚀与防护学报, 1998, 18(2): 119-125.
[10] WANG Chao LEI Sheng-bin CHEN Shen-hao YU Xi-ling (Department of Chemistry; Shandong University; Jinan; 250100). POTENTIOSTATIC CURRENT OSCILLATIONS OF IRON IN H_2SO_4 SOLUTION UNDER THE INFLUENCE OF Cl~- AND MAGNETIC FIELDS[J]. 中国腐蚀与防护学报, 1998, 18(1): 27-34.
[11] CHEN Junming LU Zhanpeng (Shanghai Institute of Metallurgy; Chinese Academy of Sciences). INSTANTANEOUS AND MEMORIAL CHARACTERISTICS OF THE EFFECT OF MAGNETIC FIELD ON POLARIZATIONBEHAVIOR OF IRON[J]. 中国腐蚀与防护学报, 1997, 17(4): 276-280.
[12] CHEN Junming LU Zhanpeng (Shanghai Institute of Metallurgy; Chinese Academy of Sciences). THE EFFECT OF MAGNETIC FIELD AND INHIBITOR ON POLARIZATION BEHAVIOR OF Fe/0.5mol· L~(-1) H_2SO_4 SYSTEMS[J]. 中国腐蚀与防护学报, 1997, 17(3): 195-202.
[13] LU Zhanpeng CHEN Junming (Shanghai Institute of Metallurgy; Chinese Academy of Sciences; Shanghai 200050). ELECTROCHEMICAL STATES AND CATHODIC POLARIZATION BEHAVIOR OF IRON IN H_2SO_4+K_2Cr_2O_7 SOLUTIONS IN THE PRESENCE OR ABSENCE OF MAGNETIC FIELD[J]. 中国腐蚀与防护学报, 1997, 17(3): 203-209.
[14] CHEN Junming LU Zhanpeng (Shanghai Institute of Metallury; Chinese Academy of Sciences). THE EFFECT OF INHIBITOR ON POLARIZATION BEHAVIOR OF Fe/0.05mol· L~(-1) HCl SYSTEM IN THE PRESENCE OR ABSENCE OFMAGNETIC FIELD[J]. 中国腐蚀与防护学报, 1997, 17(2): 135-141.
[15] LU Zhanpeng CHEN Junming (Shanghai Institute of Metallurgy; Chinese Academy of Sciences). EFFECT OF MAGNETIC FIELD AND Cl~- ON ANODIC POLARIZATION BEHAVIOR OF IRON IN NEUTRAL 0.5mol/LNa_2SO_4 SOLUTION[J]. 中国腐蚀与防护学报, 1997, 17(1): 25-30.
No Suggested Reading articles found!