Rapid Detection Methods for Sulfate-reducing Bacteria in Marine Environments

doi:10.11902/1005.4537.2019.161

Journal of Chinese Society for Corrosion and protection

2019, Vol. 39

Issue (5): 387-394 DOI: 10.11902/1005.4537.2019.161

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Rapid Detection Methods for Sulfate-reducing Bacteria in Marine Environments

QI Peng^1,^2,³,WAN Yi^1,^2,³,ZENG Yan^1,^2,³,ZHENG Laibao^1,^2,³,ZHANG Dun^1,^2,³(

)

1. Key Laboratory of Marine Environmental Corrosion and Bio-fouling, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
2. Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China
3. Open Studio for Marine Corrosion and Protection, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China

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Abstract

Sulfate-reducing bacteria (SRB) are the most corrosive and the most extensively studied corrosion related microorganisms, they are widely distributed in various marine environments. Although it has been clearly known that the status of SRB is closely related with their roles in relevant corrosion processes, however the determination of the population concentration and metabolic activity of SRB is still a big problem in the present. The development of quick and accurate detection approach could provide theoretical basis and guidance for revealing the mechanism of SRB action in the corrosion process and monitoring of microbial influenced corrosion status. This paper briefly introduces the research progress of our research group in detection of the population concentration and metabolic activity of SRB, and elaborates their advantages and disadvantages, applicable conditions and detection performance, which may provide reference for the development and practical application of detection methods for the actual situation of SRB in marine environments in the future.

Key words: sulfate-reducing bacteria metabolic activity bacterial population detection

Received: 18 September 2019

ZTFLH:

TM207

Fund: National Natural Science Foundation of China (41876101) and Young Elite Scientists Sponsorship Program by CAST(2018QNRC001)

Corresponding Authors: Dun ZHANG E-mail: zhangdun@qdio.ac.cn

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Cite this article:

QI Peng, WAN Yi, ZENG Yan, ZHENG Laibao, ZHANG Dun. Rapid Detection Methods for Sulfate-reducing Bacteria in Marine Environments. Journal of Chinese Society for Corrosion and protection, 2019, 39(5): 387-394.

URL:

https://www.jcscp.org/EN/10.11902/1005.4537.2019.161 OR https://www.jcscp.org/EN/Y2019/V39/I5/387

Fig.1 Schematic diagram (a) and detection performance (b) of bioinspired polydopamine self-excitation testing platform^[18]

Fig.2 Schematic diagram (a) and detection performance (b) of grapheme oxide marker based testing platform^[21]

Fig.3 Schematic diagram (a) and detection performance (b) of bioimprinted membrane based platform^[24]

Fig.4 Schematic diagram (a) and detection performance (b) of protease activity regulation based platform^[27]

Fig.5 Schematic diagram (a) and detection performance (b) of ZnO/ZnS nanoarray based testing platform^[32]

Fig.6 Schematic representation of detecting method of bacteria with DNA nanobarcodes-based fluorescence system (a1) and construction of DNA fluorescence nanobarcodes (a2), fluorescence imaging (b1, b2), flow cytometric scatter diagram (b3, b4) and flow cytometric monitoring performances (b5, b6) in the absence (b1, b3 and b5) and presence (b2, b4 and b6) of target DNA, respectively^[35]

Fig.7 Schematic diagram (a) and detection performance (b) of lysozyme labelled testing platform^[36]

Fig.8 Schematic diagram (a) and biofilm detection performance (b) of solid-contact sulfide selective electrode (SL_n^2- is formed by adjacent voids in the lattices of S^2- and Ag₂S)

Fig.9 Changes of SRB biofilm populations (a1~d1) and metabolic activity (a2~d2) characterized with fluorescent probes for 7 h (a1, a2), 14 h (b1, b2), 60 h (c1, c2) and 80 h (d1, d2)

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