Investigation of Microorganisms in Corrosion Product Scales on Q235 Carbon Steel Exposed to Tidal- and Full Immersion Zone at Qindao- and Sanya-sea Waters

doi:10.11902/1005.4537.2017.087

Journal of Chinese Society for Corrosion and protection

2018, Vol. 38

Issue (4): 333-342 DOI: 10.11902/1005.4537.2017.087

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Investigation of Microorganisms in Corrosion Product Scales on Q235 Carbon Steel Exposed to Tidal- and Full Immersion Zone at Qindao- and Sanya-sea Waters

Yan SUN, Jiajia WU, Dun ZHANG(

), Shiqiang CHEN

Key Laboratory of Marine Environmental Corrosion and Bio-fouling, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China

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Abstract

Q235 carbon steel samples were exposed to tidal- and full immersion-zone at two selected sea waters, namely Qingdao- and Sanya-sea waters for 90, 180 and 270 d months respectively. Then, of which the corrosion morphology, corrosion rate, and the composition of corrosion products were characterized, while the microorganisms in the rust scales were isolated, purified, and identified with the aid of the conventional plate isolation and 16S rDNA sequencing technologies. It was found that the corrosion rate of Q235 carbon steel varied with the location of the test samples and the corrosion rate of the test sample in the tidal zone was always higher than that in the immersion zone. There were variations in the composition of the inner- and outer-layer of corrosion products, and the outer consisted of Fe₃O₄, α-FeOOH, and γ-FeOOH, while Fe₃O₄ dominated in the inner layer. Furthermore, the composition of rust scales was independent on the sea waters. There was a complex microorganism community in the rust scales, which varied with the exposure sea waters and time, as well as the location of test samples. The community was comprised of sulphate-reducing bacteria, iron bacteria, and aerobic bacteria/facultative anaerobes. More species and a larger quantity of isolated bacteria were obtained for the corrosion scales formed in the immersion zone than those in the tidal zone at any exposure time, and it was more facile to detect sulphate-reducing bacteria for the corrosion scales formed in the immersion zone. Aerobic bacteria and facultative anaerobes covered diverse genera, and the dominant bacteria were Vibrio sp., Bacillus sp. and Pseudoalteromonas sp..

Key words: marine corrosion samples in natural seawater microorganism isolation and identification corrosion zone space-time variation

Received: 03 June 2017

ZTFLH:

TG174.4

Fund: Supported by National Basic Research Program of China (2014CB643304)

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Cite this article:

Yan SUN, Jiajia WU, Dun ZHANG, Shiqiang CHEN. Investigation of Microorganisms in Corrosion Product Scales on Q235 Carbon Steel Exposed to Tidal- and Full Immersion Zone at Qindao- and Sanya-sea Waters. Journal of Chinese Society for Corrosion and protection, 2018, 38(4): 333-342.

URL:

https://www.jcscp.org/EN/10.11902/1005.4537.2017.087 OR https://www.jcscp.org/EN/Y2018/V38/I4/333

Table 1 Name, composition and applications of used culture media

Fig.1 Surface morphologies of Q235 carbon steel samples immersed for 180 d in the tidal zone (a) and immersion zone (b) of Qingdao seawater

Fig.2 Surface morphologies of Q235 carbon steel samples immersed for 180 d in the tidal zone (a), and immersion zone (b) of Sanya seawater

Table 2 Average corrosion rates of Q235 carbon steel during immersion in seawater at Qingdao and Sanya for 90 and 180 d

Fig.3 XRD patterns of corrosion products formed on Q235 carbon steel after exposed to tidal (a) and immersion (b) zones of Qingdao seawater for 180 d

Fig.4 XRD patterns of corrosion products formed on Q235 carbon steel after exposed to tidal (a) and immersion (b) zones of Sanya for 180 d

Table 3 SRB detected in the rust layer formed on Q235 carbon steel exposed to tidal and immersion zones of Qingdao and Sanya seawater for different time

Table 4 IOB detected in the rust layer formed on Q235 carbon steel exposed to tidal and immersion zones of Qingdao and Sanya seawater for different time

Aerobe and facultaticve anaerobe species			Qingdao / d					Sanya / d
			Immersion zone		Tidal zone			Immersion zone							Tidal zone
			90	180	90		180	90		180			270		90		180	270
Vibrio sp.	Vibrio alginolyticus		√		√								√					√
	Vibrio parahaemolyticus		√	√						√
	Vibrio sp.		√		√								√
	Vibrio coralliilyticus							√		√
	Vibrio fortis							√		√
	Vibrio chagasii				√								√
	Vibrio diabolicus							√
	Vibrio communis							√
	Vibrio ponticus														√
	Vibrio neocaledonicus														√
	Vibrio natriegens																√
	Vibrio caribbeanicus				√
	Vibrio harveyi				√
	Vibrio brasiliensis				√
Pseudoalteromonas sp.	Pseudoalteromonas sp.		√	√	√			√		√					√		√
	Pseudoalteromonas phenolica				√			√		√
	Pseudoalteromonas luteoviolacea		√							√
	Pseudoalteromonas rubra							√		√
	Pseudoalteromonas piscicida				√					√
	Pseudoalteromonas flavipulchra														√
Bacillus sp.	Bacillus algicola						√	√		√					√		√
	Bacillus sp.														√		√
	Bacillus pumilus							√
	Bacillus anthracis							√
	Bacillus aryabhattai									√
	Bacillus cereus														√
	Bacillus methylotrophicus																√
	Bacillus aquimaris						√
Microbulbifer sp.	Microbulbifer sp.														√		√
Microbulbifer sp.	Microbulbifer agarilyticus														√		√
Alteromonas sp.	Alteromonas sp.				√										√		√
Alteromonas sp.	Alteromonas macleodii
Photobacterium sp.	Photobacterium sp.							√
	Photobacterium rosenberg							√		√
	Photobacterium lutimaris																√
Tenacibaculum sp.	Tenacibaculum discolor															√
	Tenacibaculum litoreum															√
	Tenacibaculum mesophilum															√
Erythrobacter sp.	Erythrobacter sp.	√
Erythrobacter sp.	Erythrobacter aquimaris															√
	Rhodobacteraceae					√			√		√					√
	Flavobacteriaceae													√		√
	Ruegeria atlantica													√		√
	Alpha proteobacterium													√		√
	Arthrobacter nicotianae											√
	Enterobacter sp.											√
	Phaeobacter caeruleus											√
	Rhodobacter vinaykumaraii															√
	Aquimarina latercula															√
	Thalassomonas agarivorans															√
	Acinetobacter sp.	√
	Sulfitobacter delicatus	√
	Psychrobacter adeliensis	√

Table 5 Aerobe and facultaticve anaerobe detected in the rust layer formed on Q235 carbon steel exposed to tidal and immersion zones of Qingdao and Sanya seawater for different time

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