不同海域、不同腐蚀区带Q235碳钢实海挂片腐蚀产物层内微生物调查

doi:10.11902/1005.4537.2017.087

中国腐蚀与防护学报

2018, Vol. 38

Issue (4): 333-342 DOI: 10.11902/1005.4537.2017.087

研究报告

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不同海域、不同腐蚀区带Q235碳钢实海挂片腐蚀产物层内微生物调查

孙艳, 吴佳佳, 张盾(

), 陈士强

中国科学院海洋研究所中国科学院海洋环境腐蚀与生物污损重点实验室青岛 266071

Investigation of Microorganisms in Corrosion Product Scales on Q235 Carbon Steel Exposed to Tidal- and Full Immersion Zone at Qindao- and Sanya-sea Waters

Yan SUN, Jiajia WU, Dun ZHANG(

), Shiqiang CHEN

Key Laboratory of Marine Environmental Corrosion and Bio-fouling, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China

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摘要:

将Q235碳钢样品挂于不同海域 (青岛和三亚) 的不同腐蚀区带 (潮差区和全浸区),在不同的时间点 (90,180和270 d) 取出,就腐蚀形貌、腐蚀速率、腐蚀产物成分进行分析,并采用传统平板分离和16S rDNA序列分析技术对腐蚀产物层中的微生物进行分离、纯化和培养。结果表明,Q235碳钢在不同海域不同腐蚀区带的腐蚀速率不同,且潮差区的腐蚀速率总是大于全浸区的;不同腐蚀区带腐蚀产物的内外层成分存在差别,外层含有Fe₃O₄,α-FeOOH和γ-FeOOH等,内层主要是Fe₃O₄,而不同海域形成的腐蚀产物成分差别不明显。不同时空下不同腐蚀区带Q235碳钢挂片腐蚀产物层中的细菌群落结构复杂且存在差异,主要包括硫酸盐还原菌、铁细菌和好氧/兼性厌氧菌等;在相同时空条件下,全浸区的细菌种类和数量均高于潮差区的,且更容易检测到硫酸盐还原菌的存在;好氧/兼性厌氧菌种类丰富,以弧菌属、芽孢杆菌属和假交替单胞菌属为优势菌。

关键词 ：海洋腐蚀, 实海挂样, 微生物分离, 鉴定腐蚀区带, 时空变化

Abstract：

Q235 carbon steel samples were exposed to tidal- and full immersion-zone at two selected sea waters, namely Qingdao- and Sanya-sea waters for 90, 180 and 270 d months respectively. Then, of which the corrosion morphology, corrosion rate, and the composition of corrosion products were characterized, while the microorganisms in the rust scales were isolated, purified, and identified with the aid of the conventional plate isolation and 16S rDNA sequencing technologies. It was found that the corrosion rate of Q235 carbon steel varied with the location of the test samples and the corrosion rate of the test sample in the tidal zone was always higher than that in the immersion zone. There were variations in the composition of the inner- and outer-layer of corrosion products, and the outer consisted of Fe₃O₄, α-FeOOH, and γ-FeOOH, while Fe₃O₄ dominated in the inner layer. Furthermore, the composition of rust scales was independent on the sea waters. There was a complex microorganism community in the rust scales, which varied with the exposure sea waters and time, as well as the location of test samples. The community was comprised of sulphate-reducing bacteria, iron bacteria, and aerobic bacteria/facultative anaerobes. More species and a larger quantity of isolated bacteria were obtained for the corrosion scales formed in the immersion zone than those in the tidal zone at any exposure time, and it was more facile to detect sulphate-reducing bacteria for the corrosion scales formed in the immersion zone. Aerobic bacteria and facultative anaerobes covered diverse genera, and the dominant bacteria were Vibrio sp., Bacillus sp. and Pseudoalteromonas sp..

Key words： marine corrosion samples in natural seawater microorganism isolation and identification corrosion zone space-time variation

收稿日期: 2017-06-03

ZTFLH:

TG174.4

基金资助:国家重点基础研究发展计划 (2014CB643304)

作者简介:

作者简介孙艳,女,1982年生,硕士,助理实验师

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孙艳, 吴佳佳, 张盾, 陈士强. 不同海域、不同腐蚀区带Q235碳钢实海挂片腐蚀产物层内微生物调查[J]. 中国腐蚀与防护学报, 2018, 38(4): 333-342.
Yan SUN, Jiajia WU, Dun ZHANG, Shiqiang CHEN. Investigation of Microorganisms in Corrosion Product Scales on Q235 Carbon Steel Exposed to Tidal- and Full Immersion Zone at Qindao- and Sanya-sea Waters. Journal of Chinese Society for Corrosion and protection, 2018, 38(4): 333-342.

链接本文:

https://www.jcscp.org/CN/10.11902/1005.4537.2017.087 或 https://www.jcscp.org/CN/Y2018/V38/I4/333

表1 培养基名称、成分及用途

图1 不同腐蚀区带的青岛实海挂片180 d样品的腐蚀形貌

图2 不同腐蚀区带的三亚实海挂片180 d样品的形貌

表2 青岛、三亚实海挂片90和180 d后的平均腐蚀速率

图3 Q235碳钢在青岛实海挂片180 d腐蚀产物的XRD谱

图4 Q235碳钢在三亚实海挂片180 d后腐蚀产物的XRD谱

表3 Q235碳钢在不同时空下潮差区和全浸区实海挂片锈层中SRB检测结果

表4 Q235碳钢在不同时空下潮差区和全浸区实海挂片腐蚀产物中铁细菌分离结果

Aerobe and facultaticve anaerobe species			Qingdao / d					Sanya / d
			Immersion zone		Tidal zone			Immersion zone							Tidal zone
			90	180	90		180	90		180			270		90		180	270
Vibrio sp.	Vibrio alginolyticus		√		√								√					√
	Vibrio parahaemolyticus		√	√						√
	Vibrio sp.		√		√								√
	Vibrio coralliilyticus							√		√
	Vibrio fortis							√		√
	Vibrio chagasii				√								√
	Vibrio diabolicus							√
	Vibrio communis							√
	Vibrio ponticus														√
	Vibrio neocaledonicus														√
	Vibrio natriegens																√
	Vibrio caribbeanicus				√
	Vibrio harveyi				√
	Vibrio brasiliensis				√
Pseudoalteromonas sp.	Pseudoalteromonas sp.		√	√	√			√		√					√		√
	Pseudoalteromonas phenolica				√			√		√
	Pseudoalteromonas luteoviolacea		√							√
	Pseudoalteromonas rubra							√		√
	Pseudoalteromonas piscicida				√					√
	Pseudoalteromonas flavipulchra														√
Bacillus sp.	Bacillus algicola						√	√		√					√		√
	Bacillus sp.														√		√
	Bacillus pumilus							√
	Bacillus anthracis							√
	Bacillus aryabhattai									√
	Bacillus cereus														√
	Bacillus methylotrophicus																√
	Bacillus aquimaris						√
Microbulbifer sp.	Microbulbifer sp.														√		√
Microbulbifer sp.	Microbulbifer agarilyticus														√		√
Alteromonas sp.	Alteromonas sp.				√										√		√
Alteromonas sp.	Alteromonas macleodii
Photobacterium sp.	Photobacterium sp.							√
	Photobacterium rosenberg							√		√
	Photobacterium lutimaris																√
Tenacibaculum sp.	Tenacibaculum discolor															√
	Tenacibaculum litoreum															√
	Tenacibaculum mesophilum															√
Erythrobacter sp.	Erythrobacter sp.	√
Erythrobacter sp.	Erythrobacter aquimaris															√
	Rhodobacteraceae					√			√		√					√
	Flavobacteriaceae													√		√
	Ruegeria atlantica													√		√
	Alpha proteobacterium													√		√
	Arthrobacter nicotianae											√
	Enterobacter sp.											√
	Phaeobacter caeruleus											√
	Rhodobacter vinaykumaraii															√
	Aquimarina latercula															√
	Thalassomonas agarivorans															√
	Acinetobacter sp.	√
	Sulfitobacter delicatus	√
	Psychrobacter adeliensis	√

表5 Q235碳钢实海挂片锈层优势好氧/兼性厌氧菌分离情况

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