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Journal of Chinese Society for Corrosion and protection  2018, Vol. 38 Issue (2): 174-182    DOI: 10.11902/1005.4537.2017.147
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Inhibition Effect of Pseudoalteromonas Piscicida on Corrosion of Q235 Carbon Steel in Simulated Flowing Seawater
Sai YE1,2, Moradi Masoumeh2, Zhenlun SONG2, Fangqin HU2, Zhaohui SHUN2, Jianping LONG1()
1 College of Materials and Chemistry & Chemical Engineering, Chengdu University of Technology, Chengdu 610059, China;
2 Key Laboratory of Marine New Materials and Related Technology, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo 315201, China
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Abstract  

Pseudoalteromonas piscicida was separated and extracted from seafloor sediments located in offshore waters of Zhoushan institute of marine corrosion at Zhoushan islands of the East China Sea. Then the bacterium was inculated to laboratorial bottle that placed in a constant temperature incubator shaker and to an incubator with environment of simulated lowing sea water, respectively. The effect of Pseudoalteromonas piscicida on the corrosion of Q235 carbon steel in the above two bacterial culture systems has been studied by means of electrochemical workstation, scanning electron microscope and Fourier infrared spectrometer. Results showed that this bacterium could inhibit effectively the corrosion of Q235 carbon steel in seawater. The impedance of the carbon steel was enhanced more obviously in laboratorial bottle and its surface was completely covered with an uniform and dense biofilm, while the uneven biofilm formed on the surface of carbon steel in the simulated flowing seawater system, seawater can direct contact the substrate via holes and crevices which were randomly distributed throughout the biofilm, provided conditions for the formation of oxygen concentration cell, therefore weakened the corrosion resistance of carbon steel. FT-IR spectrum showed there were differences in secretory macromolecules for the same bacterium but cultured respectively in the two culture systems after 7 d.
Key words:  Pseudoalteromonas piscicida      corrosion inhibition      laboratorial culturing      simulated marine environmental culturing      oxygen concentration cell     
Received:  09 September 2017     
Fund: Supported by National Natural Science Foundation of China (5161101078), President's Fellowship Programme of CAS (PIFI), Zhejiang Public Welfare Project (2015C31031) and Ningbo Natural Science FundProject (2015A610070)
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Articles by authors
Sai YE
Moradi Masoumeh
Zhenlun SONG
Fangqin HU
Zhaohui SHUN
Jianping LONG

Cite this article: 

Sai YE, Moradi Masoumeh, Zhenlun SONG, Fangqin HU, Zhaohui SHUN, Jianping LONG. Inhibition Effect of Pseudoalteromonas Piscicida on Corrosion of Q235 Carbon Steel in Simulated Flowing Seawater. Journal of Chinese Society for Corrosion and protection, 2018, 38(2): 174-182.

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https://www.jcscp.org/EN/10.11902/1005.4537.2017.147     OR     https://www.jcscp.org/EN/Y2018/V38/I2/174

Fig.1  Schematic diagram of work electrode
Fig.2  Diagram of laboratorial bottle culture system
Fig.3  Diagram of simulated marine environmental culture system
Fig.4  Nyquist (a~d) and Bode (e~h) plots of Q235 carbon steel immersed in LB (a, c, e, g) and FS (b, d, f, h) without (a, b, e, f) and with (c, d, g, h) bacteria
Fig.5  Equivalent circuits of EIS for Q235 carbon steel after immersion in LB and FS without and with bacteria for 0 h (a) and in LB (b) and FS (c) for 1~14 d, respectively
Condition Rs
Ωcm2		 Rct
kΩcm2		 Rof
Ωcm2		 Rb
Ωcm2		 CPEdl
μFcm-2		 CPEof
μFcm-2		 CPEb
μFcm-2
LB/0 h(sterile) 1.58 0.511 --- --- 1.9 --- ---
LB/0 h(bacterial) 3.14 2.136 --- --- 12.5 --- ---
LB/1 d(sterile) 3.39 0.794 45.6 --- 32.6 78.6 ---
LB/1 d(bacterial) 1.63 12.53 78.5 89.1 23.7 64.3 36.5
LB/3 d(sterile) 8.47 0.266 170.1 --- 31.8 98.8 ---
LB/3 d(bacterial) 7.64 12.76 265.6 212.4 56.2 67.3 76.8
LB/7 d(sterile) 2.89 0.601 335.6 --- 71.4 109.4 ---
LB/7 d(bacterial) 0.81 13.36 677.3 376.2 32.4 54.2 155.4
LB/14 d(sterile) 1.87 0.234 403.8 --- 53.2 67.6 ---
LB/14 d(bacterial) 2.31 28.96 765.2 579.6 36.6 121.7 96.8
FS/0 h(sterile) 3.91 0.975 --- --- 4.6 --- ---
FS/0 h(bacterial) 3.23 1.843 --- --- 6.7 --- ---
FS/1 d(sterile) 4.29 0.422 54.3 --- 22.7 65.3 ---
FS/1 d(bacterial) 5.45 2.264 32.3 56.2 16.2 77.6 22.9
FS/3 d(sterile) 3.23 0.608 107.4 --- 63.2 89.2 ---
FS/3 d(bacterial) 2.87 2.449 49.3 128.3 10.5 34.6 13.4
FS/7 d(sterile) 2.13 0.701 154.2 --- 67.4 83.5 ---
FS/7 d(bacterial) 1.12 5.122 78.8 107.6 17.3 28.9 56.7
FS/14 d(sterile) 6.65 0.495 275.4 --- 43.9 90.7 ---
FS/14 d(bacterial) 3.45 8.743 72.5 316.5 16.6 43.8 49.5
Table.1  Impedance parameters of Q235 carbon steel after immersion in LB and FS without and with bacteria for different time
Fig.6  Polarization curves of Q235 carbon steel immersed in LB (a) and FS (b) with and without bacteria for 1 and 14 d (b), and in LB and FS with bacteria for 1, 7 and 14 d (c)
Condition EcorrV Icorr
μAcm-2		 βc
mVdec-1		 βa
mVdec-1		 Rp
kΩcm2		 Corrosion rate
mma-1
LB/1 d(sterile) -0.928 26.74 -78.8 99.2 0.19 2.87
LB/1 d(bacterial) -0.907 35.91 -69.6 176.5 0.36 1.89
LB/7 d(bacterial) -0.821 11.62 -75.9 189.6 1.19 0.27
LB/14 d(sterile) -0.866 39.92 -86.9 114.6 0.52 1.96
LB/14 d(bacterial) -0.714 4.16 -92.3 191.3 2.45 0.09
FS/1 d(sterile) -0.885 56.38 -43.4 89.6 0.46 3.23
FS/1 d(bacterial) -0.913 49.33 -366.3 120.1 0.24 3.54
FS/7 d(bacterial) -0.905 31.62 -39.2 115.8 0.78 2.79
FS/14 d(sterile) -0.984 55.81 -75.8 176.2 0.28 2.15
FS/14 d(bacterial) -0.815 11.67 -54.1 166.4 1.67 0.97
Table 2  Potentiodynamic polarization parameters of Q235 carbon steel after immersion in LB and FS for different time
Fig.7  SEM images of Q235 carbon steel after immersion in LB (a, b, e, f) and FS (c, d, g, h) for 7 d (a~d) and 14 d (e~h)
Fig.8  SEM images (a, c) and corresponding EDS results (b, d) of Q235 carbon steel immersed in LB (a, b) and FS (c, d) for 7 d
Fig.9  FT-IR spectra of the biofilms formed on Q235 carbon steel after immersion in LB and FS contains artificial seawater with bacteria for 7 d (a) and 14 d (b)
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